monoclonal mouse anti cd68/sr-d1  (R&D Systems)

Journal: Frontiers in Immunology

Article Title: The CXCL9/SPP1 polarity axis in tumor-associated macrophages: immunoregulatory and prognostic significance in non-small cell lung cancer

doi: 10.3389/fimmu.2026.1763652

Figure Lengend Snippet: Validation of CXCL9 + and SPP1 + macrophage expression and polarization characteristics in lung cancer tissues. (A) Representative mIF staining for CXCL9+ TAMs and SPP1+ TAMs in lung tissue sections from the normal and LUAD tumor groups. DAPI (blue), CD68 (red), CXCL9 (yellow), SPP1 (green) are shown, along with individual and merged channels. (n = 3 per group). Scale bar, 20 μm. (B) Representative mIF staining of CXCL9+ TAMs (yellow) and SPP1+ TAMs (green) in lung tissue sections from the normal and LUAD tumor groups (n = 3 per group). Scale bar, 20 μm. (C) qRT-PCR validation of macrophage polarization. The M1 group showed high expression of Cxcl9 and iNOS, while the M2 group showed high expression of Spp1 and Arg1. Asterisks indicate statistically significant differences (*p < 0.05).

Article Snippet: Following another stripping step, the third round was performed using mouse anti-human CD68 monoclonal antibody (1:50000, Servicebio, Cat# GB153150 ) with HRP-conjugated goat anti-mouse secondary antibody (ready-to-use, Servicebio, Cat# G1301), and signal was developed using iF555-Tyramide (1:500, Servicebio, Cat# G1233).

Techniques: Biomarker Discovery, Expressing, Staining, Quantitative RT-PCR

Journal: Cancer Research Communications

Article Title: Phase I First-in-Human Study of TRK-950, an IgG1 Antibody Specific to CAPRIN-1, in Patients with Advanced Solid Tumors

doi: 10.1158/2767-9764.CRC-25-0123

Figure Lengend Snippet: Graphs depicting the density of macrophage subsets pre- and posttreatment with TRK-950. Tumor specimens obtained with core-needle biopsies at the screening and C1D22 time points were subjected to multi-immunofluorescence to detect macrophage infiltrates. Quantitative analyses were performed on digital images of CD68 and CD163 staining. A, total CD68 + cells; ( B ) CD68 + /CD163 − subsets; ( C ) CD68 + /CD163 + subsets. Error bars indicate SD. C1D22, cycle 1 day 22; SCR, screening.

Article Snippet: After antigen retrieval (36 minutes at 95°C, pH 8.4), 4-μm-thick sections of tumor specimens obtained from core-needle biopsies at the screening and C1D22 time points were stained with mouse monoclonal anti-human CD68 (IgG2b, clone 298807, R&D Systems) and mouse monoclonal anti-human CD163 (IgG1, clone 10D6, Leica Biosystems) and then incubated with AF647 goat anti-mouse IgG2b and AF488 goat anti-mouse IgG1 (both from Invitrogen).

Techniques: Immunofluorescence, Staining

Journal: Frontiers in Immunology

Article Title: Activated hepatic stellate cell-derived small extracellular vesicles facilitate M2 macrophage polarization and hepatoma progression via miR-27a-3p

doi: 10.3389/fimmu.2024.1489679

Figure Lengend Snippet: miR-27a-3p promotes the M2 polarization of macrophage. (A) Representative images for the immunohistochemical staining of macrophage maker CD68 and CD206 in nude mice xenografts, bar = 100 μm; and the number of CD68, CD206 positive cells per 200 × field for each group (mean ± SEM). **P < 0.01, ***P < 0.001 vs control. Below each group of images is an image showing the magnification of the corresponding box area above. (B) The expression of miR-27a-3p in mimic-miR-27a-3p or anti-miR-27a-3p treated THP1 cells was evaluated by qRT-PCR 24 h after transfection, normalized to U6 snRNA (mean ± SEM). **P < 0.01, ****P < 0.0001 vs. control. (C, D) The expression of CD206 was evaluated in mimic-miR-27a-3p or anti-miR-27a-3p treated THP1 cells by western blotting normalized to β-actin (mean ± SEM). *P < 0.05, ****P < 0.0001 vs. control. The data were from three independent experiments.

Article Snippet: Monoclonal antibodies against CD206 (Boster, China), CD68 (Boster, China), Ki67 (Abcam, USA), and SPRY2 (Proteintech, China) were used.

Techniques: Immunohistochemical staining, Staining, Control, Expressing, Quantitative RT-PCR, Transfection, Western Blot

Journal: eLife

Article Title: Human-induced pluripotent stem cell-derived microglia integrate into mouse retina and recapitulate features of endogenous microglia

doi: 10.7554/eLife.90695

Figure Lengend Snippet:

Article Snippet: Antibody , anti-human CD68 (mouse monoclonal) , R&D , Cat. #: MAB20401 , IHC (1:100).

Techniques: Virus, Recombinant, In Situ, Plasmid Preparation, cDNA Synthesis, SYBR Green Assay, Flow Cytometry, Staining, Lysis, Bicinchoninic Acid Protein Assay, Software

Journal: bioRxiv

Article Title: Transglutaminase 2 function in glioblastoma tumor efferocytosis

doi: 10.1101/2024.08.29.610293

Figure Lengend Snippet: A and B. TGM2 RNA expression in glioblastoma molecular subtypes in the TCGA and CGGA datasets; C and D. Correlation of TGM2 mRNA levels with mRNA levels for the macrophage/microglia marker CD68 in the TCGA and CGGA datasets; E. The fifty genes showing the highest positive Spearman correlation with TGM2 mRNA expression in the TCGA database were identified using cBioPortal. This gene set was then analyzed for matches to cell type datasets. Yellow highlights show the match to macrophage cell type identified using Enrichr under Cell Types/ARCHS4 Tissues (34/50 match, qval = 2.8×10-15, odds ratio 5.35); F. TGM2 mRNA expression in single cell RNAseq data. Plots show UMAP clustering using data from Abdelfattah et al . analyzed with the Broad Institute Single Cell Portal. Plots show cell type assignments (left) and TGM2 mRNA expression (right); G. Violin plots (with all data points) of TGM2 mRNA expression in glioblastoma tumour cell types; H. Violin plots (with all data points) of TGM2 mRNA in the glioblastoma tumour immune cell subtypes described by Abdelfattah et al.

Article Snippet: Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts) and anti-CD68 mouse monoclonal antibody (used for immunofluorescence in human tissue samples) were from Millipore/Sigma (cat. #s MABN92 and AMAB90873, respectively).

Techniques: RNA Expression, Marker, Expressing

Journal: bioRxiv

Article Title: Transglutaminase 2 function in glioblastoma tumor efferocytosis

doi: 10.1101/2024.08.29.610293

Figure Lengend Snippet: A. Image from full section showing strong TGM2 expression near regions of necrosis (indicated with N). Regions without necrosis (lower left) show lower levels of TGM2 staining; B. Necrotic region showing TGM2 positive cells surrounding the border of the necrotic region, similar to what was observed in the xenograft model. C-F. Double immunofluorescence for CD68 and TGM2 in whole sections from patient tumours. For each image row, the left panel shows CD68, the middle panel shows TGM2, and the right panel shows the merged image. Except for E, each row shows a single focal plane image from the Z stack. Row C shows a region with macrophages and endothelial cells. Row D shows a higher magnification view of a tumour blood vessel with adjacent macrophages to illustrate differences in subcellular location. Row E shows a Z stack of the same region as B, emphasizing the co-localization of TGM2 and CD68 in macrophages. Row F shows an area adjacent to necrotic regions.

Article Snippet: Anti-Iba1/AIF1 mouse monoclonal antibody (used for immunofluorescence in xenografts) and anti-CD68 mouse monoclonal antibody (used for immunofluorescence in human tissue samples) were from Millipore/Sigma (cat. #s MABN92 and AMAB90873, respectively).

Techniques: Expressing, Staining, Immunofluorescence